mRNA can be synthesized by in vitro transcription (IVT) with the help of RNA polymerase. IVT mRNA is very sensitive to degradation by nucleases, which limits its suitability for transfections and therapeutic applications. The poly(A)-tail and the cap structure contribute to the stability of the mRNA. The 5’- and 3’-UTRs include specific regulatory sequences that are necessary to modulate the stability and translation of the mRNA. The protein-coding region of the mRNA can be optimized to improve the translation of the desired protein and to resist for the endonucleolytic degradation.
One of the challenges of mRNA medicine is to optimize the mature mRNA chain for target specific delivery for cells or tissues. We produce the core materials used for capping, tailing, and sequence optimization already. We are capable of screening the targeted mRNA in short time with unbeatable low cost.