mRNA is considered as a difficult material to handle with for a long time, because it can be degraded by the RNase in the environment quickly. Manufacturing the standardized mRNA in large quantity and high quality is the first challenge in the whole process.
The modification of the nucleotides, the capping and tailing of mRNA play key roles in mRNA stability and half-life in cytosol. High-throughput screening is required for high efficiency and specificity of protein translation. Packaging and delivery mRNA into cell or tissue is also an essential step needed to optimize.
Therefore, the mRNA medicine development requires a group of scientists from multidisciplinary fields, Including but not limited to the combination of pathology, physiology, chemistry, and materials.
Nature Reviews Drug Discovery volume17, pages261–279 (2018)